Direct Coombs test (gel method) (Direct Antiglobulin Test, DAT)
Diagnosis of immune forms of hemolytic anemia. Detection of antibodies or complement components fixed on the erythrocyte surface. Used when autoimmune hemolysis, post-transfusion reactions, and hemolytic disease of the newborn are suspected.
What the test evaluates:
- Presence of IgG immunoglobulins and/or complement components on erythrocytes
- Immune mechanism of erythrocyte destruction
Biomaterial: Venous blood
- No special preparation required
Medications:
- Immunoglobulins, hormonal and immunosuppressive therapy may affect results
- Inform physician of all medications taken
Recommended for:
- Suspected autoimmune hemolytic anemia
- Complications after blood transfusion
- Risk or signs of hemolytic disease of the newborn
- Anemia of unclear origin, jaundice, elevated bilirubin
Important: Positive result indicates presence of antibodies or complement on erythrocytes and confirms immune mechanism of hemolysis. Final diagnosis and treatment determined by physician considering clinical picture and other lab data.
