DNA Ancylostoma duodenale (hookworm) — PCR, qualitative
Detects Ancylostoma duodenale DNA — roundworm causing hookworm disease. PCR detects genetic material even when eggs/larvae destroyed.
Infection routes:
- contaminated soil or sand;
- contact with infected earth;
- poor hygiene;
- unwashed vegetables, fruits, water.
Recommended for:
- persons with chronic anemia or intestinal symptoms;
- soil/agricultural workers;
- elevated eosinophils;
- negative microscopy results.
Biomaterial: Stool
- Do not take laxatives 2-3 days before
- Do not use enemas or rectal suppositories
- Collect stool naturally; exclude urine and water contamination
- Use sterile container; collect from 3-5 different areas
- Deliver within 2-3 hours; store at +2 to +8°C up to 8-12 hours if needed
Medications: If taking antiparasitic drugs — inform physician; collect before treatment if possible
Note: PCR testing after treatment is usually done no earlier than 2-3 weeks after.
- Abdominal pain;
- anemia, weakness, fatigue;
- nausea, reduced appetite;
- diarrhea or constipation;
- unexplained allergic reactions;
- elevated eosinophils;
- suspected parasitic infection;
- negative microscopy with symptoms.
Note: Test detects even small parasite DNA fragments.
Negative — A. duodenale DNA not detected.
Positive — A. duodenale DNA detected; confirms parasite presence.
Physician consultation required at positive result.
Infectious disease parasitologist consultation recommended. Self-treatment not recommended.
