DNA Dicrocoelium dendriticum (lancet liver fluke) — PCR, qualitative
Detects Dicrocoelium dendriticum DNA — a parasitic trematode (liver fluke) causing dicrocoeliosis. PCR detects genetic material even when eggs/larvae are destroyed and undetectable by microscopy. More sensitive than microscopy.
Infection routes:
- consuming poorly cooked products contaminated with parasite larvae;
- contact with contaminated soil or water;
- poor hygiene.
Recommended for:
- persons with chronic liver/gallbladder diseases;
- prolonged digestive disorder symptoms;
- negative microscopy results;
- elevated blood eosinophils.
Biomaterial: Stool
- Do not take laxatives 2-3 days before
- Do not use enemas or rectal suppositories
- Collect stool naturally; exclude urine and water contamination
- Use sterile container; collect from 3-5 different areas
- Deliver within 2-3 hours; store at +2 to +8°C up to 8-12 hours if needed
Medications: If taking antiparasitic drugs — inform physician; collect before treatment if possible
Note: PCR testing after treatment usually not earlier than 2-3 weeks.
- Right upper quadrant pain;
- nausea, reduced appetite;
- digestive disorders, abdominal bloating;
- enlarged liver and lymph nodes;
- unexplained allergic reactions;
- suspected parasitic infection;
- negative microscopy with symptoms.
Note: Test detects even small parasite DNA fragments with proper sample collection.
Negative — DNA not detected.
Positive — DNA detected; confirms parasite presence.
Physician consultation required at positive result.
Infectious disease parasitologist consultation recommended. Self-treatment not recommended.