DNA Opisthorchis viverrini — PCR, qualitative
Detects Opisthorchis viverrini DNA — a parasitic trematode (fluke) causing opisthorchiasis. Parasite affects bile ducts, liver, and pancreas. PCR detects genetic material even when eggs/larvae destroyed.
Infection routes:
- consuming raw, lightly salted, dried, or poorly cooked river fish;
- cross-contamination of kitchen equipment.
Recommended for:
- river fish consumers;
- endemic region residents;
- chronic liver/gallbladder diseases;
- elevated eosinophils;
- prolonged digestive disorders;
- negative microscopy results.
Biomaterial: Stool
- Do not take laxatives 2-3 days before
- Do not use enemas or rectal suppositories
- Collect stool naturally; exclude urine and water contamination
- Use sterile container; collect from 3-5 different areas
- Deliver within 2-3 hours; store at +2 to +8°C up to 8-12 hours if needed
Medications: If taking antiparasitic drugs — inform physician; collect before treatment if possible
Note: PCR testing after treatment is usually done no earlier than 2-3 weeks after.
- Right upper quadrant pain;
- nausea, bitter taste;
- bloating, digestive disorders;
- unstable stool;
- unexplained allergic reactions;
- elevated eosinophils;
- liver enlargement;
- chronic bile duct diseases;
- suspected parasitic infection;
- negative microscopy with symptoms.
Note: Test detects even small parasite DNA fragments.
Negative — O. viverrini DNA not detected.
Positive — O. viverrini DNA detected; confirms parasite presence.
Physician consultation required at positive result.
Infectious disease parasitologist consultation recommended. Self-treatment not recommended.
