DNA Trichinella spiralis — PCR, qualitative
Detects Trichinella spiralis DNA — roundworm causing trichinellosis. PCR detects genetic material even when eggs/larvae destroyed.
Infection routes:
- consuming poorly cooked meat (pork, game);
- improper meat storage;
- raw/home-made sausage.
Recommended for:
- persons consuming home or game pork;
- symptoms of muscle pain, edema, weakness;
- negative microscopy with symptoms.
Biomaterial: Stool
- Do not take laxatives 2-3 days before
- Do not use enemas or rectal suppositories
- Collect stool naturally; exclude urine and water contamination
- Use sterile container; collect from 3-5 different areas
- Deliver within 2-3 hours; store at +2 to +8°C up to 8-12 hours if needed
Medications: If taking antiparasitic drugs — inform physician; collect before treatment if possible
Note: PCR testing after treatment is usually done no earlier than 2-3 weeks after.
- Muscle pain and cramps;
- face and eyelid edema;
- elevated temperature;
- weakness, fatigue;
- nausea, vomiting;
- allergic manifestations;
- suspected parasitic infection;
- negative microscopy with symptoms.
Note: Test detects even small parasite DNA fragments.
Negative — T. spiralis DNA not detected.
Positive — T. spiralis DNA detected; confirms parasite presence.
Physician consultation required at positive result.
Infectious disease parasitologist consultation recommended. Self-treatment not recommended.
