Immunoblot for autoimmune diabetes type I-II — GAD, IA-2, IAA, ICA, ZnT8
Detects autoantibodies to key pancreatic antigens; determines autoimmune diabetes origin and differentiates type 1 from type 2.
Profile (immunoblot, quantitative): GAD (anti-glutamate decarboxylase); IA-2 (anti-tyrosine phosphatase); IAA (anti-insulin autoantibodies); ICA (anti-islet cell antibodies); ZnT8 (anti-zinc transporter 8)
Deviation mechanism: antibodies attack beta-cells → reduced insulin production → elevated glucose.
Biomaterial: Venous blood
- Draw blood fasting (8-12 hours without food)
- Exclude alcohol 24 hours before
- Avoid stress and physical activity 30 minutes before
- Early or atypical diabetes onset;
- unexplained hyperglycemia;
- suspected autoimmune beta-cell damage;
- diabetes type differential diagnosis.
Negative — autoantibodies not detected.
Positive — antibodies to pancreatic antigens detected; confirms autoimmune diabetes.
Results evaluated with clinical picture and laboratory data.
Endocrinologist consultation recommended.
